What Are Reagents

Reagents are substances or compounds added to initiate or accelerate the reaction.

There are several types of reagents that are used in molecular biology processes.

Polymerase Chain Reaction (PCR)

Real-Time PCR (qPCR),

RT-PCR RNA and

DNA purification Genotyping Protein biology Gel Electrophoresis

Which are usually used with Agarose, DNA ladders and staining dyes to analyze your PCR results.

Lab Reagents come from  different manufacturers such as Lambda Biotech, Frogga Bio and Benchmark Accuris. Agarose can offer a range of options like Low Melt, High Resolution and more.

What Are Some of the Popular Lab Reagents?

One of the popular brands is Lamda Biotech. Lamda Biotech Reagent products range from basic culture media, DNA/protein ladders to real-time PCR and cell transfection, have been tested on the market for over twenty years. They are essential products for all life science research labs. Lamda Biotech products are backed by the highest quality control standard and with high-level live technical support.

What Is a Popular PCR Reagent?

Lamda Biotech Lab PCR Reagents are the most popular reagent. The Lamda Bio Taq DNA Polymerase has the most popular thermostable enzyme used in DNA amplification experiments. This high-performance Taq DNA Polymerase is specially purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the most economic DNA polymerases available. SPECIAL FEATURES Robust performance Leaves 3’A overhang Stable at all storage temperatures Best price/quality APPLICATIONS General PCR Genomic analysis TA cloning

What are popular DNA & RNA Purification Reagent Systems?

Lamda Bio Column-Pure Plasmid Mini-Prep Kit. The most widely used kit in modern molecular biology laboratories; this kit utilizes a silica spin filter to purify plasmid DNA. It is the easiest method for isolation of plasmid DNA and produces high-yield plasmid DNA.

The recovered plasmid DNA has a 1.8-2.0 OD260/280 ratio and is ready for such downstream applications as automated sequencing, and restriction digests.

The purified plasmid DNA is primarily in the supercoiled form.

SPECIAL FEATURES

10-minute,

easy-to-follow method

Consistent results Best price/quality

KIT CONTENTS

Solution I

Solution II

Solution III

Wash Solution

Elution Buffer

Spin Columns

RNase A

Protocol Manual

What is Direct PCR & Genotyping?

A Genotyping Kit contains all the reagents needed for quick extraction and direct amplification of genomic DNA from cells, mouse tails and other animal tissues; such as liver, muscle, hair shafts, skin and saliva. TheLamda Bio DIRECT-PCR KIT: For Mouse Genotyping, 1000rxn provides these to several labs.

The kit contains everything needed and is optimized for working with animal genomic DNA.

-The PCR product can be directly loaded onto agarose gel for electrophoresis, without adding loading dye.

-The kit makes genotyping quicker and easier, especially if you have a large number of samples.

SPECIAL FEATURES: Easy: no DNA purification needed Consistent: containing pre-optimized 2X PCR mix Specificity: little or no background Sensitivity: work with low amount of.

DNA APPLICATIONS: Genotyping – widely used for genotyping Mutation detection Target detection in transgenic mice Quantitative PCR, DNA sequencing and cloning Can be easily adapted to high throughput system; such as Qiagen and other company’s automation systems

What is Agarose gel electrophoresis?

Agarose gel electrophoresis is used to separate DNA by size or topology using an electric field that induces negatively charged DNA molecules to migrate to the positive pole through a porous matrix of agarose. Agarose (gel) is a polysaccharide, generally extracted from certain red seaweed. It is a linear polymer made up of the repeating unit of agarobiose, which is a disaccharide made up of D-galactose and 3,6-anhydro-L-galactopyranose.PCR Reagents include Taq Polymerase, Master Mix, Genotyping kits,cDNA synthesis kits, DNA stains, DNA Ladders and Agarose. Benchmark Accuris Taq DNA Polymerase offers higher solubility and template affinity, resulting in consistently superior performance.

What is one of the Top Agarose Gels?

Lamda Bio Agarose. This is a biotechnology grade, standard gelling temperature agarose. This agarose is most commonly found in research laboratories and it is useful for many protocols in molecular biology, especially for separation of bands larger than 500bp. Other applications include: analytical and preparatory electrophoresis of DNA, Northern and Southern blotting, PCR analysis and immuno assays. Each batch of agarose is functionally tested in molecular biology applications. Unlike other agaroses, this agarose will not boil out of the container.

What Is A Reagent Reservoir?

Multichannel Reagent Reservoirs and Dual Solution Basins Reagent Reservoirs Multi-channel pipettes are perfect for powering through large sets of pipetting, but easily aspirating the solution can be difficult without a reagent or solution reservoir. Designed to reduce reagent waste, many reagent reservoirs come with a ‘V’ shaped bottom and spouts at the corner to help pour the solution out when finished. Sturdy and disposable, most solution reservoirs are made of polystyrene and come in size ranges of 10 mL to 100 mL. Reagent Reservoirs features and benefits Solution reservoirs have a variety of different features and benefits, depending on the format you pick, to meet the specific needs that the reservoir was designed for. These are just some of the features and benefits of popular models below: Reagent Divides Lids Easy Storage Indented “Spouts” for Easy Pour Perfect 4, 8, & 12 Channel Pipettes