What is A Fixed Volume Pipette

A fixed volume pipette, also known as a fixed-volume micropipette, is a laboratory instrument used for precise and accurate measurement and transfer of liquid volumes. It is commonly used in various scientific disciplines such as biology, chemistry, and molecular biology.

Unlike adjustable or variable volume pipettes, which allow users to set and change the desired volume, fixed volume pipettes have a pre-set, non-adjustable volume. These pipettes are designed to deliver a specific and consistent volume with each use.

Fixed volume pipettes are available in a range of volume capacities, typically ranging from microliters (μL) to milliliters (mL). They are designed with a piston mechanism and a disposable tip that can be attached and detached for each use, ensuring accurate and contamination-free transfers.

Fixed volume pipettes are widely used in research laboratories, diagnostic laboratories, pharmaceutical industries, and other scientific settings where precise and repetitive liquid handling is required.

A fixed volume pipette and an automatic pipette differ in several key aspects:

  1. Volume Adjustability: One of the primary differences is in the volume adjustability. A fixed volume pipette has a pre-set, non-adjustable volume. It can deliver only a specific volume with each use, and the volume cannot be changed. On the other hand, an automatic pipette offers adjustable volume settings. Users can set and change the desired volume within the pipette’s range.
  2. Mechanism: Fixed volume pipettes typically have a manual operating mechanism. They are manually operated by the user, who aspirates and dispenses the liquid by applying pressure on the plunger. In contrast, automatic pipettes have an electronic mechanism. They feature a motorized system that aspirates and dispenses the liquid with the push of a button, reducing the manual effort required.
  3. Automation and Features: Automatic pipettes offer additional automation features not found in fixed volume pipettes. These features may include programmable functions such as multiple dispensing, mixing, and serial dilutions. Automatic pipettes can also have advanced functions like sequential dispensing, reverse pipetting, and customizable pipetting protocols. These automated features enhance efficiency, accuracy, and reproducibility in liquid handling tasks.
  4. Display and Controls: Fixed volume pipettes usually have a basic design with minimal controls. They may have a simple scale or indicator to show the fixed volume. In contrast, automatic pipettes typically have a digital display and control panel. The digital display allows users to see the set volume and other relevant information, while the control panel enables users to adjust the volume, select functions, and navigate through menu options.
  5. User Experience: Fixed volume pipettes require the user to manually control and perform all pipetting actions, including aspirating, dispensing, and discarding tips. Automatic pipettes, with their electronic mechanism, simplify the pipetting process. They offer features like automatic tip ejection, minimizing the need for manual tip handling. This can enhance user experience and reduce repetitive strain during prolonged pipetting sessions.

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