Universal Pipette Tip vs a Filtered Pipette Tip

Universal pipette tips and filtered pipette tips are both commonly used in laboratories for various pipetting applications. While they serve similar purposes, there are differences between the two pipette tips in terms of their design and functionality.

  1. Universal Pipette Tips: Universal pipette tips are the standard type of tips used with pipettes. They are typically made of polypropylene and have a straight, open-ended design. Universal tips are used for general pipetting tasks where sample contamination is not a major concern. They are suitable for applications such as transferring liquids between containers, dilutions, and general pipetting in molecular biology, biochemistry, and other fields.
  2. Filtered Pipette Tips: Filtered pipette tips, also known as barrier tips, are designed with an additional filtration component to prevent contamination of the pipette and the sample being pipetted. The filter is usually made of hydrophobic material, such as porous polyethylene, that allows air to pass through but blocks liquids and aerosols. This filter acts as a barrier, preventing any liquid or microbial contaminants from reaching the pipette shaft.

    Filtered tips are particularly useful when working with sensitive samples, such as DNA, RNA, PCR reactions, cell cultures, or any application where contamination can affect the experimental results. The filtration component helps to minimize the risk of cross-contamination between samples and protects the pipette from potentially harmful substances. Filtered tips are commonly used in molecular biology, microbiology, and clinical laboratories.

It’s important to note that filtered tips are not necessary for all applications. If your work involves non-sensitive samples or if contamination is not a major concern, universal tips can be a cost-effective choice. However, when working with precious or potentially contaminated samples, using filtered tips is recommended to ensure the integrity of your experiments.

In summary, while both universal and filtered pipette tips serve the purpose of transferring liquids, filtered tips provide an additional layer of protection against contamination and are ideal for applications where sample purity is critical. The choice between the two depends on the specific requirements of your experiment and the level of sample protection needed.


What is the most important aspect of a pipette tip?

The most important aspect of a pipette tip is its ability to accurately and precisely deliver the desired volume of liquid during pipetting. Here are some key considerations for an effective pipette tip:

  1. Accuracy: The tip should be designed to provide accurate volume measurements, ensuring that the intended volume is transferred without significant variation or error.
  2. Precision: The tip should allow for precise delivery of liquid, minimizing any variation or deviation from the target volume.
  3. Compatibility: The tip should be compatible with the specific pipette being used to ensure a secure fit and prevent leakage or liquid loss during pipetting.
  4. Contamination prevention: The tip should be designed to prevent cross-contamination between samples, minimizing the risk of carryover or contamination that could affect experimental results.
  5. Ergonomics: The tip should be easy to attach and eject from the pipette, facilitating efficient and comfortable pipetting.
  6. Material quality: The tip material should be of high quality, free from impurities that could interfere with experimental procedures or introduce contaminants into samples.
  7. Aerosol reduction: Some tips are designed to minimize the generation of aerosols during pipetting, reducing the risk of sample loss or exposure to hazardous substances.
  8. Graduations and markings: Clear and accurate volume markings on the tip can aid in precise pipetting, especially when handling small volumes.
  9. Racks and packaging: The design of the tip racks and packaging should allow for easy access, storage, and organization, ensuring convenience and minimizing the risk of contamination or damage.
  10. Cost-effectiveness: While not the most critical aspect, the cost-effectiveness of the tips is a consideration, particularly when large quantities are needed for routine use.

Considering these factors, selecting high-quality pipette tips that meet the specific requirements of the experiment or application is crucial for accurate and reliable pipetting.

Can a Lab use the same Pipette Tip for an experiment?

In general, it is not recommended to reuse pipette tips between experiments. Pipette tips are designed to be disposable and single-use items for several reasons:

  1. Contamination: Reusing pipette tips increases the risk of cross-contamination between samples, which can lead to inaccurate and unreliable results. Even small traces of previous samples or reagents left in the tip can affect the integrity of your experiment.
    1. Using a contaminated pipette tip can have several negative consequences for your experiment:
      1. Cross-contamination: Contaminants present in the pipette tip can transfer to subsequent samples or reagents, leading to cross-contamination. This can result in false positive or false negative results, compromising the accuracy and reliability of your experiment.
      2. Experimental integrity: Contaminants in the pipette tip can introduce unwanted variables into your experiment. They may interact with your samples or reagents, potentially altering their composition or behavior and leading to misleading or erroneous results.
      3. Inconsistent measurements: Contaminants in the pipette tip can affect the volume or concentration of the liquid being dispensed. This can lead to inconsistent or inaccurate measurements, making it difficult to reproduce or compare your results.
      4. Invalidation of results: Contamination from previous samples or reagents can render your experiment invalid. If you are working with sensitive or low-concentration samples, even trace amounts of contaminants can have a significant impact on your results, making them uninterpretable or unreliable.

      To ensure the integrity and accuracy of your experiments, it is crucial to use clean and uncontaminated pipette tips for each sample or reagent. Disposable, single-use pipette tips are specifically designed to minimize the risk of contamination and ensure consistent and reliable measurements.

  2. Accuracy: Pipette tips are calibrated for specific volumes, and their accuracy can be compromised if they are reused. Residue from previous samples or reagents may remain in the tip, affecting the volume delivered and leading to imprecise measurements.
  3. Convenience: Using fresh pipette tips for each experiment allows for a more efficient workflow. It saves time by eliminating the need for cleaning and ensuring that you always have a clean, reliable tip available.

While there may be situations where reusing pipette tips is permissible, such as when transferring the same solution repeatedly within the same experiment, it is generally best practice to use a new, sterile pipette tip for each sample or reagent.

What are the best pipette tips for your lab?

The choice of pipette tips for your lab depends on various factors, including the specific applications, the type of pipettes being used, and any special requirements you may have. However, we can provide you with an overview of some popular pipette tip options commonly used in labs:

  1. Universal Pipette Tips: These tips are designed to fit a wide range of pipettes from different manufacturers. They are compatible with both single-channel and multi-channel pipettes, offering versatility and convenience.
  2. Filtered Pipette Tips: These tips contain built-in filters that provide an additional barrier to prevent aerosol and liquid contamination from entering the pipette shaft. They are particularly useful when working with samples that are sensitive to contamination, such as PCR or DNA/RNA amplification assays.
  3. Low-Retention Pipette Tips: These tips are specially designed to minimize sample retention on the inner walls of the tip. They help ensure accurate and precise dispensing of liquid, especially for samples with high viscosity or low surface tension. Low-retention tips are commonly used in applications like protein handling, genomics, and cell culture.
  4. Extended Length Pipette Tips: These tips have longer and narrower shafts, which are beneficial when pipetting from deep, narrow containers, such as microcentrifuge tubes or deep-well plates. They enable reaching the bottom of the vessels without the need for additional manual steps.
  5. Specialty Pipette Tips: Depending on your lab’s specific requirements, you may need specialty pipette tips such as wide-bore tips for pipetting viscous liquids, conductive tips for use in electrostatic discharge (ESD) protected areas, or sterile tips for aseptic work.

It’s always a good practice to consult with your lab members or supervisor to determine the most suitable pipette tips for your specific applications. Additionally, considering factors like tip quality, availability, and cost can help you make an informed decision.

The Importance of Choosing the Right Racked Pipette Tips

In the world of scientific research, precision and accuracy are paramount. One crucial element that often goes overlooked is the selection of the right racked pipette tips for experiments. These small accessories can have a significant impact on the outcome of your research, affecting accuracy, precision, and contamination prevention. In this blog post, we will explore the importance of choosing the appropriate racked pipette tips for your experiments and how they can significantly enhance the reliability and reproducibility of your results.

  1. Accuracy Matters: Accurate measurements are the foundation of any scientific experiment. Choosing the right racked pipette tips that are compatible with your pipette model ensures proper alignment and calibration, leading to accurate volume delivery. Ill-fitting or inferior quality tips can compromise the precision of your pipetting, resulting in inaccurate measurements and potentially invalidating your experimental results.
  2. Precision for Reliable Results: Precision is another critical factor in scientific experiments. Racked pipette tips that offer consistent and reproducible performance help minimize variations between samples and replicate measurements. The uniformity in tip design, including the inner diameter and shape, plays a crucial role in ensuring precise liquid transfers. By selecting high-quality tips, you can reduce the risk of systematic errors and improve the reliability of your data.
  3. Contamination Prevention: Contamination is a persistent concern in laboratory settings. Racked pipette tips that are designed to prevent contamination are essential for maintaining the integrity of your experiments. Filtered tips, for instance, incorporate filters that prevent aerosols, liquids, or particulate matter from entering the pipette or being transferred to subsequent samples. This feature is particularly crucial when working with sensitive samples, such as DNA or RNA, where even minute contamination can have significant consequences.
  4. Sample Retention and Low-Retention Tips: Certain experiments involve working with valuable or limited samples. In such cases, minimizing sample loss is of utmost importance. Low-retention racked pipette tips are specifically designed to reduce sample retention, ensuring maximum recovery of precious samples. These tips utilize advanced surface technologies to minimize liquid adhesion, preventing sample loss and resulting in more accurate measurements.
  5. Specialty Tips for Specific Applications: Different experiments may require specialized racked pipette tips tailored to specific applications. Extended length tips, for instance, are ideal for reaching deep wells or tubes, ensuring efficient and precise liquid transfers. Similarly, specialty tips designed for gel loading, PCR, or cell culture provide optimal performance and compatibility for their respective applications. By selecting the right tips for your specific needs, you can enhance the efficiency and accuracy of your experiments.

When it comes to scientific research, every detail matters. The choice of racked pipette tips may seem insignificant compared to the complexity of experiments, but it has a profound impact on accuracy, precision, and contamination prevention. By investing in high-quality tips that are compatible with your pipette model and tailored to your specific application, you can significantly improve the reliability and reproducibility of your results. So, next time you embark on an experiment, don’t underestimate the importance of choosing the right racked pipette tips—it could be the key to unlocking accurate and meaningful scientific discoveries


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Should I Use Compatible Pipette Tips

While the majority of users buy LTS tips from Rainin due to not knowing there are alternatives, Pipette.com is here to provide a solution. Rainin LTS are high quality, but also come with a high price tag. Manufactured here in the United States with 100% medical grade virgin polypropylene, Pipette.com offers Rainin LTS compatible pipette tips for use on all “L” model Rainin pipettes.

Pipette.com has all volume ranges from 10 uL up to 1200 uL, selling the LTS tips in all packaging and sterility formats. This includes bulk bags, reload stacks, racked sterile, filtered/barrier, and low retention. Producing an airtight seal during aspiration, these Pipette.com LTS pipette tips for Rainin ” model pipettes are ideal for maintaining consistent measurements with no loss of sample.

If these Compatitable Pipette Tips don’t work for your lab. another great option are:

Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips are the ideal aid for all your pipetting needs. Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips are standard multipurpose tips that are designed for various lab applications. Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips are high-quality standard pipette tips and are an excellent solution for any lab. Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips (non-filter) are available at various purity levels and packaging options and are suitable for use with other manufacturers’ pipettes. Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips are fully autoclavable at 121°C for 20 minutes with 1 bar/100 kPa pressure.

Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips are designed and manufactured to perfectly fit Sartorius pipettes, enabling maximal accuracy and precision when used together. However, the universal design also secures compatibility with most other pipette makes and models on the market today. To protect your sample from contamination, Sartorius Pipette Tips such as the Optifit Easybulk Pipette Tips are manufactured in a protected ISO 8 Clean Room environment. The packaging is all also 100% recyclable, producing the minimum amount of waste.

Exploring the Best Pipette Tips on the Market: Enhancing Accuracy and Efficiency in the Lab

In the world of scientific research and laboratory work, precision and accuracy are paramount. One critical tool that directly influences the accuracy of liquid handling is the pipette tip. With numerous brands and options available, it can be challenging to determine which pipette tips are the best fit for your experiments. In this blog, we will delve into the top pipette tips on the market that are renowned for their quality, performance, and reliability, helping you make an informed choice for your laboratory needs.

  1. Eppendorf Tips:

Eppendorf is a well-established brand known for its high-quality laboratory products. Their pipette tips are designed with precision in mind. The tips exhibit excellent accuracy and precision, ensuring reliable and reproducible results. Eppendorf tips are available in various sizes, including low-retention and filter options, to accommodate diverse experimental requirements. Their ergonomic design and robust construction make them user-friendly and resistant to contamination or sample loss.

  1. CAPP Pipette Tips:

CAPP Line of Expell & Expell Plus Pipette tips provides some of the highest quality Low Retention Filter Tips and Non-Filtered Tips on the market. Expell Plus tips deliver volumes within 0.1% of the set volume, versus 0.5-0.7% for standard tips. All tips are designed with a softer collar to ensure an airtight seal for the widest range of pipettes. The low binding properties of CAPP tips are achieved without the use of additives to the plastic used in the tips, preserving the integrity of your samples and preventing contamination. Ideal for volumes below 1 mL, the CAPP ExpellPlus pipette tips provide extreme precision.

  1. Oxford Lab Products Tips:

Oxford Lab Products Universal Pipette Tips are certified DNase, RNase, Human DNA, Pyrogen, and PCR inhibitor-free, ideally suited for highly sensitive materials and procedures such as PCR laboratory operations. Not only are they certified free of all those contaminants, these tips are also free from lubricants and heavy metals which can often ruin the integrity of samples during aspiration and dispensing. Featuring an innovative design, the universal pipette tips are built for adaptability and compatibility with a wide variety of pipettes from several different manufacturers. Built to have low force requirements for pipette tip loading and tip ejecting, these universal pipette tips are ergonomically designed to prevent injuries within the lab stemming from repeated movements and force.

  1. Sartorius Pipette Tips:

In liquid handling, pipetting results are not dependent on the pipette or the tip alone, but also on the combination of them both together and the quality of their fit and seal. Sartorius’ Optifit Tips and SafetySpaceTM Filter Tips are Sartorius Tips designed and manufactured to perfectly fit Sartorius pipettes, enabling maximal accuracy and precision. The Sartorius Tips’ universal design also secures compatibility with most other pipette makes and models on the market toda

  1. BRAND Pipette Tips:

BRAND is a renowned manufacturer of laboratory consumables, including pipette tips. Their tips are engineered with attention to detail, focusing on precision and accuracy. BRAND tips offer a perfect seal, preventing sample loss and contamination. They are designed to be compatible with multiple pipette models, providing flexibility and convenience in the laboratory. BRAND also offers specialty tips for specific applications, such as extended length tips for deep-well plates or wide-bore tips for viscous samples.


Choosing the best pipette tips for your laboratory work is crucial to ensure accurate and reliable results. The brands mentioned above, including Eppendorf, CAPP, Oxford, Thermo Scientific, and BRAND, have established themselves as leaders in the field, offering high-quality pipette tips that enhance accuracy, precision, and efficiency in liquid handling. By selecting reputable brands and considering factors such as tip design, compatibility, and application-specific features, you can make an informed decision that aligns with your experimental requirements. Investing in top-quality pipette tips will contribute to the success of your research, promoting reliable and reproducible data in the laboratory.